2012-07-01·European archives of oto-rhino-laryngology : official journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery3区 · 医学

Tissue repair driven by two different mechanisms of growth factor plasmids VEGF and NGF in mice auricular cartilage: regeneration mediated by administering growth factor plasmids

3区 · 医学

Article

作者: Daniela Pinterova ; Martin Cegan ; Katarina Kolostova ; Lenka Ceganova ; Vladimir Bobek ; Oliver Taltynov ; Marie Jirkovska

The focus of this study was to compare the role of nerve growth factor (NGF) and vascular endothelial growth factor (VEGF) in the regeneration of experimental skin and cartilage trauma. The role of VEGF in this process is known since decade; the NGF participation on this process has been first discussed within the spinal cord injury repair. We hypothesized that both VEGF and NGF induce angiogenesis and take part on the repair process. The angiogenesis response and the cartilage regeneration after phVEGF(165) plasmid and rat pcNGF plasmid administration were investigated using BALB/c mice. PhVEGF(165) and pcNFG were injected into the right mice ear and plain vector injection into the left ear the day before trauma. The next day, all mice were ear-punched, resulting in 2-mm diameter puncture through the center of both pinnae. In BALB/c mouse strain, a significantly faster cartilage repair was observed after phVEGF(165) and pcNGF injection into punched ear area in comparison to the control group. It has been shown that the healing process is after VEGF and NGF injection driven differentially. In case of VEGF is the cartilage wound repaired by induction of new chondrocytes differentiation. In the case of NGF, the regeneration is supported by immature leukocytes attracted into the punched area. The leukocytes induct angiogenesis so far indirectly by inflammation. The NGF-induced inflammation environment may be a part of mosaic creating the complete picture of regeneration.

2008-10-01·Diabetic medicine : a journal of the British Diabetic Association2区 · 医学

Skin capillary permeability in the diabetic foot with critical limb ischaemia: the effects of a phVEGF165 gene product

2区 · 医学

Article

作者: J. H. Van Der Hoeven ; N. H. Mulder ; Y. Kusumanto ; A. J. Smit ; K. De Leeuw ; G. A. P. Hospers ; P. Oomen

Abstract:

Aims To measure capillary permeability, assessed by skin capillary sodium fluorescein (NaF) leakage, in patients with diabetes mellitus with critical limb ischaemia (DM‐CLI) and to compare the effects of vascular endothelial growth factor (VEGF) with those of placebo.Methods NaF leakage was assessed in 17 patients with DM‐CLI, in 24 diabetes mellitus (DM) patients without clinical signs of macrovascular disease or neuropathy (DM‐C) and in 22 healthy control subjects. The 17 DM‐CLI patients were randomized to receive phVEGF165 gene product (n = 11) or placebo (n = 6). Measurements were repeated after 28 days.Results DM‐CLI patients had a longer dye arrival time (DAT), but NaF leakage was similar to control subjects, while capillary permeability was increased in DM‐C compared with control subjects. Leakage curve rose in patients receiving VEGF and fell in those receiving placebo, 28 days after administration. The decrease in DAT in the VEGF group was not significant, whilst DAT rose in the placebo group. Perfusion pressures were similar in the two groups.Conclusion No increase in capillary leakage in DM‐CLI was found, probably because an increased capillary filtration coefficient is counterbalanced by a marked fall in perfusion pressures. Increased capillary leakage may be one explanation for oedema formation after VEGF treatment.

2007-04-01·Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban

Favorable effect of local VEGF gene injection on axonal regeneration in the rat sciatic nerve

Article

作者: Fu, Chongyang ; Hong, Guangxiang ; Wang, Fabin

This study is to investigate the effect of local phVEGF(165) injection on sciatic nerve regeneration in the rats and to search for a new way in the further treatment of peripheral nerve injuries. Forty-five adult male Wistar rats received a neurotomy to bilateral sciatic nerves, which were subsequently reconnected with 10/0 epineurial nylon sutures. The injured segments was locally injected with normal saline (group A), or 25 microg of phVEGF(165) (group B) or 50 microg phVEGF(165) (group C). Nerve conduction and regeneration were evaluated in terms of the histological changes, weight of gastrocnemius muscles, electrophysiology and morphometric results. Our study demonstrated that rats of group C showed the best results in terms of nerve regeneration, followed by group B and group A. Our findings suggested that local injection of phVEGF165 can facilitate nerve regeneration and promote functional recovery in a dose-dependent manner.

100 项与 VEGF-2 gene therapy(Steward St. Elizabeth's Medical Center of Boston, Inc.) 相关的药物交易

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适应症	最高研发状态	国家/地区	公司	日期
糖尿病性神经病变	临床2期	-	Steward St. Elizabeth's Medical Center of Boston, Inc.	-
糖尿病性神经病变	临床2期	-	Northwest University	-

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研究	分期	人群特征	评价人数	分组	结果	评价	发布日期


Phase III Comparison of Intramuscular Delivery of Naked Plasmid Encoding Vascular Endothelial Growth Factor with Placebo in Diabetic Patients with Critical Limb Ischaemia (ASGCT2002)	临床3期	慢性肢体威胁性缺血 diabetic retinopathy \| history of malignancy	-	Naked Plasmid Encoding Vascular Endothelial Growth Factor (phVEGF165)	夢餘膚網襯窪鬱淵築壓(願選憲齋網選構壓艱鹹) = Complications were limited to transient lower extremity edema in three patients 網襯襯膚淵鏇鏇鑰餘構 (鏇憲鑰糧餘襯衊憲顧繭 ) 更多	积极	2002-05-01